Basmalah, Vryzas Ramadhan Bara (2026) Pengaruh Variasi Konsentrasi Cu dan Temperatur Kalsinasi terhadap Karakteristik Borate-Based Bioactive Glass Menggunakan Metode Spray Drying untuk Aplikasi Bone Filler. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 5011221121-Undergraduate_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
5011221121-Undergraduate_Thesis.pdf Restricted to Repository staff only Download (11MB) | Request a copy |
Abstract
Material bone filler perlu memiliki kemampuan antibakteri karena infeksi bakteri dapat menghambat proses penyembuhan tulang. Penelitian ini bertujuan untuk menganalisis pengaruh variasi konsentrasi tembaga (Cu) dan temperatur kalsinasi terhadap karakteristik borate-based bioactive glass (BBG) yang disintesis menggunakan metode spray drying. Variasi konsentrasi Cu yang digunakan adalah 1, 3, dan 5 mol%, sedangkan temperatur kalsinasi divariasikan pada 500, 600, dan 700°C. Karakterisasi dilakukan melalui analisis visual, X-Ray Fluorescence (XRF), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), SEM–Energy Dispersive X-ray (SEM–EDX), Thermogravimetric Analysis–Differential Scanning Calorimetry (TGA–DSC), uji aktivitas antibakteri terhadap Escherichia coli dan Staphylococcus aureus, serta uji viabilitas sel osteoblas menggunakan MTT assay. Peningkatan konsentrasi Cu menyebabkan warna sampel semakin kebiruan dan meningkatkan kandungan Cu yang terdeteksi melalui SEM–EDX. Hasil XRD menunjukkan bahwa sampel yang dikalsinasi pada 500°C masih didominasi struktur amorf, sedangkan peningkatan temperatur hingga 600 dan 700°C meningkatkan pembentukan fasa kristalin dan aglomerasi partikel. Pada variasi Cu 3 mol%, kehilangan massa menurun dari 16,66% pada Cu3T500 menjadi 5,15% pada Cu3T600 dan 1,76% pada Cu3T700. Temperatur puncak endotermik berada pada rentang 331,07–344,86°C. Uji antibakteri menunjukkan bahwa Cu3T600 menghasilkan zona hambat sebesar 21,5 mm terhadap E. coli dan 26,5 mm terhadap S. aureus. Seluruh sampel menunjukkan viabilitas sel osteoblas sebesar 79,70–90,86% selama pengamatan 24, 48, dan 72 jam, sehingga tidak menunjukkan efek sitotoksik dan mendukung sitokompatibilitas in vitro pada kondisi pengujian. Meskipun Cu3T700 memiliki kehilangan massa paling rendah dan Cu5T600 menghasilkan viabilitas sel tertinggi, Cu3T600 menunjukkan keseimbangan paling baik antara karakteristik material, aktivitas antibakteri, dan viabilitas sel osteoblas sehingga berpotensi dikembangkan sebagai kandidat BBG untuk aplikasi bone filler antibakteri.
=======================================================================================================================================
Bone filler materials require antibacterial properties because bacterial infection can interfere with the bone-healing process. This study aimed to analyze the effects of copper (Cu) concentration and calcination temperature on the characteristics of borate-based bioactive glass (BBG) synthesized using the spray drying method. The Cu concentrations were varied at 1, 3, and 5 mol%, while the calcination temperatures were set at 500, 600, and 700°C. Characterization was performed using visual analysis, X-Ray Fluorescence (XRF), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), SEM–Energy Dispersive X-ray (SEM–EDX), Thermogravimetric Analysis–Differential Scanning Calorimetry (TGA–DSC), antibacterial testing against Escherichia coli and Staphylococcus aureus, and osteoblast cell viability testing using the MTT assay. Increasing the Cu concentration produced a more bluish sample color and increased the Cu content detected by SEM–EDX. XRD analysis showed that samples calcined at 500°C remained predominantly amorphous, whereas increasing the calcination temperature to 600 and 700°C promoted crystalline phase formation and particle agglomeration. At a Cu concentration of 3 mol%, the mass loss decreased from 16.66% in Cu3T500 to 5.15% in Cu3T600 and 1.76% in Cu3T700. The endothermic peak temperatures ranged from 331.07 to 344.86°C. Antibacterial testing showed that Cu3T600 produced inhibition zones of 21.5 mm against E. coli and 26.5 mm against S. aureus. All samples exhibited osteoblast cell viability values of 79.70–90.86% during the 24-, 48-, and 72-hour observation periods, indicating that the samples did not exhibit cytotoxic effects and supported in vitro cytocompatibility under the testing conditions. Although Cu3T700 exhibited the lowest mass loss and Cu5T600 produced the highest cell viability, Cu3T600 demonstrated the best balance among material characteristics, antibacterial activity, and osteoblast cell viability, indicating its potential as a BBG candidate for antibacterial bone filler applications.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Borate-based bioactive glass, Cu, spray drying, kalsinasi, antibakteri, viabilitas sel, bone filler. borate-based bioactive glass, Cu, spray drying, calcination, Antibacterial activity, cell viability, bone filler. |
| Subjects: | R Medicine > R Medicine (General) R Medicine > R Medicine (General) > R857 Biomedical materials--Effect of lasers on |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Vryzas Ramadhan Bara Basmalah |
| Date Deposited: | 24 Jul 2026 02:09 |
| Last Modified: | 24 Jul 2026 02:09 |
| URI: | http://repository.its.ac.id/id/eprint/137242 |
Actions (login required)
 |
View Item |