Kajian Respon Fisiologi Dan Molekuler Gen CSLA3 Dan SuSy2 Tanaman Amorphophallus Muelleri var. Madiun 1 (Porang) Terhadap Cekaman Pb(NO3)2

Kaamila, Dinda Najwa (2026) Kajian Respon Fisiologi Dan Molekuler Gen CSLA3 Dan SuSy2 Tanaman Amorphophallus Muelleri var. Madiun 1 (Porang) Terhadap Cekaman Pb(NO3)2. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Amorphophallus muelleri (Porang) merupakan salah satu tanaman hutan di Indonesia dengan kandungan glukomanan mencapai 70–90%. Tingginya minat porang mendorong pemanfaatan lahan suboptimal seperti lahan pasca tambang untuk meningkatkan hasil produksi. Akumulasi timbal pada tanaman dapat berdampak pada mekanisme fisiologis maupun molekuler. Penelitian ini bertujuan untuk memahami mekanisme adaptasi dan toleransi tanaman porang pada cekaman logam berat Pb(NO3)2. Metode penelitian diawali dengan budidaya tanaman porang selama 134 hari, kemudian diberikan perlakuan cekaman Pb(NO₃)₂. Paramater fisiologis yang diamati meliputi kadar klorofil, laju transpirasi, nilai Net Assimilation Rate (NAR), serta kadar glukomanan. Analisis respon molekuler dilakukan menggunakan teknik RT-PCR untuk mengevaluasi tingkat ekspresi gen CSLA3 dan SuSy2. Penelitian disusun menggunakan rancangan acak lengkap (RAL) satu faktor dengan konsentrasi 0 ppm, 150 ppm, 300 ppm, 450 ppm, dan 600 ppm. Data dianalisis dengan ANOVA satu arah dengan uji lanjut Tukey HSD. Hasil penelitian menunjukkan bahwa tanaman Amorphophallus muelleri var. Madiun 1 menunjukkan respons adaptif terhadap cekaman Pb(NO₃)₂ sehingga mampu mempertahankan toleransi terhadap kondisi cekaman logam berat. Respons tersebut ditunjukkan melalui perubahan parameter fisiologis, meliputi peningkatan kadar klorofil total, kadar glukomanan, dan Net Assimilation Rate (NAR), serta penurunan laju transpirasi. Pada tingkat molekuler, toleransi tanaman didukung oleh peningkatan ekspresi gen SuSy2 dan CSLA3 yang berperan dalam biosintesis glukomanan,sehingga mengindikasikan adanya mekanisme adaptasi fisiologis dan molekuler dalam merespons cekaman Pb(NO₃)₂.
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Amorphophallus muelleri (porang) is one of Indonesia's forest plants with a glucomannan content ranging from 70–90%. The increasing demand for porang has encouraged the utilization of suboptimal lands, such as post-mining areas, to enhance its production. However, lead (Pb) accumulation in plants may adversely affect both physiological and molecular mechanisms. This study aimed to investigate the adaptation and tolerance mechanisms of porang under Pb(NO₃)₂-induced heavy metal stress. The experiment began with cultivating porang plants for 134 days, followed by the application of Pb(NO₃)₂ treatments. The physiological parameters observed included chlorophyll content, transpiration rate, Net Assimilation Rate (NAR), and glucomannan content. Molecular responses were analyzed using Reverse Transcription Polymerase Chain Reaction (RT-PCR) to evaluate the expression levels of the CSLA3 and SuSy2 genes. The experiment was arranged in a completely randomized design (CRD) with a single factor consisting of five Pb(NO₃)₂ concentrations: 0, 150, 300, 450, and 600 ppm. Data were analyzed using one-way analysis of variance (ANOVA), followed by Tukey's Honestly Significant Difference (HSD) test. Amorphophallus muelleri var. Madiun 1 exhibited an adaptive response to Pb(NO₃)₂ stress, indicating its tolerance to heavy metal stress conditions. This response was reflected by changes in physiological parameters, including increased total chlorophyll content, glucomannan content, and net assimilation rate (NAR), as well as a decreased transpiration rate. At the molecular level, plant tolerance was supported by the upregulation of the SuSy2 and CSLA3 genes involved in glucomannan biosynthesis, indicating the presence of both physiological and molecular adaptation mechanisms in response to Pb(NO₃)₂ stress.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Gene Expression Pb(NO₃)₂ Stress, Molecular Response, Physiological Response, Porang.
Subjects:	Q Science Q Science > QH Biology Q Science > QK Botany Q Science > QK Botany > QK710 Plant physiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Dinda Najwa Kaamila
Date Deposited:	27 Jul 2026 01:03
Last Modified:	27 Jul 2026 01:03
URI:	http://repository.its.ac.id/id/eprint/137818

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