Pengaruh Pretreatment Pada Sintesis Hydroxyapatite Dari Tulang Bovine Terhadap Morfologi Dan Karakteristik Untuk Aplikasi Medis

Andrianti, Ananda Aisyah Anastasia (2025) Pengaruh Pretreatment Pada Sintesis Hydroxyapatite Dari Tulang Bovine Terhadap Morfologi Dan Karakteristik Untuk Aplikasi Medis. Other thesis, Institut Teknologi Sepuluh Nopember.

Text 5011221003_Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (5MB) | Request a copy

Abstract

Limbah tulang sapi berpotensi dimanfaatkan sebagai sumber kalsium alami untuk menghasilkan hydroxyapatite biogenik sebagai kandidat biomaterial medis. Pemanfaatan sumber biogenik ini menjadi penting karena hydroxyapatite memiliki komposisi yang menyerupai mineral utama tulang dan gigi, sehingga berpotensi digunakan dalam aplikasi regenerasi jaringan tulang. Penelitian ini bertujuan menganalisis pengaruh variasi pretreatment terhadap karakteristik hydroxyapatite dari tulang bovine serta mengevaluasi kelayakannya sebagai seed pada proses Seed Mediated Growth. Variasi penelitian meliputi jenis tulang kortikal dan trabekular, metode degreasing menggunakan etanol serta kombinasi etanol–n-heksana, temperatur kalsinasi, dan holding time. Karakterisasi dilakukan melalui analisis yield, morfologi, komposisi unsur, gugus fungsi, distribusi ukuran partikel, struktur kristal, serta biokompatibilitas. Hasil penelitian menunjukkan bahwa variasi pretreatment memengaruhi jumlah produk, morfologi, rasio Ca/P, ukuran partikel, dan kristalinitas hydroxyapatite. Tulang trabekular menghasilkan karakteristik yang lebih baik dibandingkan tulang kortikal, terutama dari aspek homogenitas morfologi, distribusi ukuran partikel, dan kesesuaian rasio Ca/P. Sampel TE91 menunjukkan karakteristik paling optimal dengan rasio Ca/P sebesar 1,72, Z-Average 183,63 nm, PDI 0,203, serta fasa hydroxyapatite yang sesuai dengan standar yang telah ditentukan. Setelah digunakan pada proses Seed Mediated Growth, TE91-SMG menghasilkan peningkatan massa dengan yield 1983,33% dan tetap menunjukkan pembentukan fasa hydroxyapatite, meskipun kristalinitas menurun menjadi 43,02%. Berdasarkan hasil tersebut, TE91 dinilai sebagai sampel paling potensial sebagai seed hydroxyapatite biogenik untuk pengembangan biomaterial medis berbasis tulang bovine.
=============================================================================================================================
Bovine bone waste has the potential to be utilized as a natural calcium source for producing biogenik hydroxyapatite as a candidate material for medical biomaterials. The use of this biogenik source is important because hydroxyapatite has a composition similar to the main mineral component of human bones and teeth, making it promising for bone tissue regeneration applications. This study aims to analyze the effect of pretreatment variations on the characteristics of hydroxyapatite derived from bovine bone and to evaluate its feasibility as a seed in the Seed Mediated Growth process. The research variations included cortical and trabecular bone types, degreasing methods using ethanol and ethanol–n-hexane, calcination temperature, and holding time. Characterization was carried out through yield analysis, morphology, elemental composition, functional groups, particle size distribution, crystal structure, and biocompatibility. The results showed that pretreatment variations affected the product yield, morphology, Ca/P ratio, particle size, and crystallinity of hydroxyapatite. Trabecular bone produced better characteristics than cortical bone, particularly in terms of morphological homogeneity, particle size distribution, and Ca/P ratio suitability. Sample TE91 showed the most optimal characteristics, with a Ca/P ratio of 1.72, Z-Average of 183.63 nm, PDI of 0.203, and a hydroxyapatite phase corresponding to the previously has been determined. After being used in the Seed Mediated Growth process, TE91-SMG showed an increase in product mass with a yield of 1983.33% and maintained the formation of the hydroxyapatite phase, although its crystallinity decreased to 43.02%. Based on these results, TE91 is considered the most potential sample as a biogenik hydroxyapatite seed for the development of bovine bone-based medical biomaterials.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bovine, Hidroksiapatit biogenik, Pre treatment. Biogenic hydroxyapatite, Bovine, Pretreatment.
Subjects:	Q Science > QH Biology > QH541.15.T68 Toxicity testing
Divisions:	Faculty of Industrial Technology > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis
Depositing User:	Ananda Aisyah Anastasia Andrianti
Date Deposited:	27 Jul 2026 02:17
Last Modified:	27 Jul 2026 02:45
URI:	http://repository.its.ac.id/id/eprint/137821

Actions (login required)

View Item