Parakassi, Muhammad Fajar (2026) Analisis Pengaruh Geometri Elektroda, Tegangan, dan Laju Aliran Fluida Terhadap Efisiensi dan Puritas Pemisahan Sel Kanker Paru (A549) Berbasis Dielektroforesis pada Sistem Mikrofluida. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 5001221039-Undergraduate_thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
5001221039-Undergraduate_thesis.pdf - Accepted Version Restricted to Repository staff only Download (2MB) | Request a copy |
Abstract
Kanker paru merupakan salah satu jenis kanker dengan angka kejadian dan mortalitas tertinggi di dunia. Sel kanker yang terlepas dari tumor primer dapat memasuki aliran darah sebagai Circulating Tumor Cells (CTCs), sehingga keberadaannya berpotensi dimanfaatkan untuk diagnosis dan pemantauan penyakit secara non-invasif. Namun, jumlah CTC yang sangat sedikit dibandingkan sel darah normal menjadi tantangan dalam proses isolasi. Penelitian ini bertujuan menganalisis pengaruh variasi geometri elektroda, tegangan elektroda, dan rasio laju aliran fluida terhadap performa pemisahan sel kanker paru A549 dari sel darah normal menggunakan metode dielektroforesis (DEP) pada sistem mikrofluida. Penelitian dilakukan melalui simulasi numerik menggunakan perangkat lunak COMSOL Multiphysics dengan geometri elektroda persegi, segitiga, dan setengah lingkaran. Variasi tegangan yang digunakan untuk geometri elektroda persegi, segitiga dan setengah lingkaran sebesar 2–8 V, sedangkan rasio laju aliran sampel dan buffer divariasikan menjadi 1:1, 1:2, dan 1:3 pada frekuensi operasi 200 kHz. Hasil penelitian menunjukkan bahwa geometri elektroda memengaruhi distribusi medan listrik dan lintasan sel. Kondisi optimum diperoleh pada elektroda segitiga dengan tegangan 7 V dan rasio laju aliran 1:2. Pada kondisi tersebut diperoleh efisiensi pemisahan sebesar 73,33% dan puritas sebesar 98,84%, dengan 22 dari 30 sel A549 berhasil dipisahkan ke outlet target tanpa kontaminasi sel darah normal.
=======================================================================================================================================
Lung cancer is a type of cancer with the highest incidence and mortality rates in the world. Cancer cells released from the primary tumor can enter the bloodstream as Circulating Tumor Cells (CTCs), so their presence has the potential to be used for non-invasive diagnosis and monitoring of disease. However, the very small number of CTCs compared to normal blood cells is a challenge in the isolation process. This study aims to analyze the effect of variations in electrode geometry, electrode voltage, and fluid flow rate ratio on the performance of separating A549 lung cancer cells from normal blood cells using the dielectrophoresis (DEP) method in a microfluidic system. The research was carried out through numerical simulations using COMSOL Multiphysics software with square, triangular and semicircular electrode geometries. The voltage variations used were 2 – 8 V for square, triangular and semicircular electrode geometries, while the sample and buffer flow rate ratios were varied to 1:1, 1:2, and 1:3 at an operating frequency of 200 kHz. The results show that electrode geometry influences the electric field distribution and cell trajectory. Optimum conditions were obtained on a triangular electrode with a voltage of 7 V and a flow rate ratio of 1:2. Under these conditions, a separation efficiency of 73.33% and purity of 98,84% was obtained, with 22 out of 30 A549 cells successfully separated into the target outlet without contamination of normal blood cells.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Dielektroforesis, Mikrofluida, A549, Faktor Clausius–Mossotti, Pemisahan Sel, Dielectrophoresis, Microfluidics, A549, Clausius–Mossotti Factor, Cell Separation |
| Subjects: | Q Science > QC Physics > QC1 Dielectrics Q Science > QC Physics > QC585 Dielectrics |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Physics > 45201-(S1) Undergraduate Thesis |
| Depositing User: | Muhammad Fajar Parakassi |
| Date Deposited: | 30 Jul 2026 01:02 |
| Last Modified: | 30 Jul 2026 01:02 |
| URI: | http://repository.its.ac.id/id/eprint/139898 |
Actions (login required)
 |
View Item |