Studi Kinetika Biodekolorisasi Metil Oranye Oleh Bakteri Pseudomonas Aeruginosa Yang Terimobilisasi Pada Matriks SA/PVA/Karbon Aktif

Izzananto, Muhammad Daffa (2026) Studi Kinetika Biodekolorisasi Metil Oranye Oleh Bakteri Pseudomonas Aeruginosa Yang Terimobilisasi Pada Matriks SA/PVA/Karbon Aktif. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Metil oranye (MO) merupakan salah satu pewarna azo yang banyak digunakan dalam berbagai industri dan memiliki sifat toksik serta sulit terdegradasi secara alami akibat struktur kimianya yang stabil. Penelitian ini bertujuan untuk mengkaji kinetika, isoterm, dan termodinamika biodekolorisasi MO menggunakan bakteri Pseudomonas aeruginosa yang terimobilisasi pada matriks SA/PVA/karbon aktif. Bakteri diimobilisasi pada matriks SA/PVA/karbon aktif dengan metode crosslinking menggunakan CaCl₂. Proses biodekolorisasi dilakukan dengan variasi waktu kontak 1–240 menit, konsentrasi awal MO 25–300 mg/L, dan suhu 30, 37, dan 45°C. Konsentrasi MO dianalisis menggunakan spektrofotometer UV-Vis, sedangkan karakterisasi karbon aktif dan beads dilakukan menggunakan X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR) serta Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX). Hasil penelitian menunjukkan bahwa waktu kesetimbangan adsorpsi tercapai pada 180 menit. Analisis kinetika menunjukkan bahwa proses adsorpsi mengikuti model pseudo orde dua dengan nilai koefisien determinasi (R²) sebesar 0,9944. Studi isoterm menunjukkan bahwa proses adsorpsi mengikuti model Langmuir dengan kapasitas adsorpsi maksimum sebesar 0,215 mg/g. Hasil studi termodinamika menunjukkan nilai energi bebas Gibbs (ΔG) pada suhu 30, 37, dan 45°C berturut-turut sebesar −2567,97; −2824,51; dan −3249,78 J/mol, yang menunjukkan bahwa proses adsorpsi berlangsung secara spontan. Nilai perubahan entalpi (ΔH) sebesar 11,24 kJ/mol mengindikasikan bahwa proses adsorpsi bersifat endotermik, sedangkan nilai perubahan entropi (ΔS) sebesar 45,50 J/mol·K menunjukkan meningkatnya ketidakteraturan sistem selama proses adsorpsi.
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Methyl orange (MO) is one of the most widely used azo dyes in various industries and is recognized for its toxicity and resistance to natural degradation due to its stable chemical structure. This study aimed to investigate the kinetics, adsorption isotherm, and thermodynamics of MO biodecolorization using Pseudomonas aeruginosa immobilized in a SA/PVA/activated carbon matrix. The bacteria were immobilized within the SA/PVA/activated carbon matrix through CaCl₂-induced crosslinking. Biodecolorization experiments were conducted by varying the contact time (1–240 min), initial MO concentration (25–300 mg/L), and temperature (30, 37, and 45°C). The residual MO concentration was determined using UV–Visible spectrophotometry, while the activated carbon and immobilized beads were characterized using X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy coupled with Energy-Dispersive X-ray Spectroscopy (SEM–EDX). The results showed that adsorption equilibrium was achieved after 180 min. Kinetic analysis revealed that the adsorption process was best described by the pseudo-second-order model, with a coefficient of determination (R²) of 0.9944. The adsorption isotherm was best fitted by the Langmuir model, yielding a maximum adsorption capacity of 0.215 mg/g. Thermodynamic analysis showed that the Gibbs free energy (ΔG) values at 30, 37, and 45°C were −2567.97, −2824.51, and −3249.78 J/mol, respectively, indicating that the adsorption process occurred spontaneously. Furthermore, the positive enthalpy change (ΔH) of 11.24 kJ/mol indicated that the adsorption process was endothermic, while the positive entropy change (ΔS) of 45.50 J/mol·K suggested an increase in the randomness of the system during the adsorption process.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi, Imobilisasi, Kinetika, Metil Oranye, Pseudomonas aeruginosa, Biodecolorization, Imobilization, Kinetics, Methyl Orange, Pseudomonas aeruginosa
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD1 Oxidation-reduction reaction. Q Science > QD Chemistry > QD117 Absorption Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Muhammad Daffa Izzananto
Date Deposited:	31 Jul 2026 07:27
Last Modified:	31 Jul 2026 07:27
URI:	http://repository.its.ac.id/id/eprint/140055

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