Peningkatan Stabilitas Nano-Trimetil trisindolin melalui Modifikasi Permukaan Gelatin Sapi dan Ikatan Silang Tripolifosfat

Wati, Dwi Ayu Sekar (2026) Peningkatan Stabilitas Nano-Trimetil trisindolin melalui Modifikasi Permukaan Gelatin Sapi dan Ikatan Silang Tripolifosfat. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Trimetil trisindolin merupakan senyawa dengan potensi aktivitas antikanker. Namun, penerapannya masih terbatas karena selektivitasnya yang rendah. Salah satu pendekatan untuk meningkatkan selektivitas adalah penggunaan sistem penghantaran obat berbasis nanoteknologi, khususnya nanodispersi dengan ukuran partikel 10–200 nm. Meskipun memiliki berbagai keunggulan, sistem nanodispersi sering mengalami masalah ketidakstabilan akibat agregasi partikel di lingkungan biologis. Dalam penelitian ini, turunan trimetil trisindolin yaitu 3,3-di(1-methyl-1H-indole-3-yl)-1-methylindolin-2-one digunakan sebagai senyawa uji. Tujuan penelitian ini adalah meningkatkan stabilitas nanodispersi trimetil trisindolin dengan memodifikasi permukaan menggunakan gelatin sapi. Gelatin dipilih karena sifatnya yang biokompatibel dan biodegradable, serta umum digunakan dalam formulasi farmasi. Proses cross-linking dengan tripolifosfat diterapkan untuk meningkatkan stabilitas struktur, dan pengaruh pH. Nanodispersi disiapkan menggunakan metode represipitasi dan dikarakterisasi berdasarkan ukuran partikel menggunakan particle size analyzer dan morfologinya menggunakan field-emission scanning electron microscopy (FESEM). Hasil penelitian menunjukkan bahwa kombinasi gelatin sapi konsentrasi 250 µg/mL dan tripolifosfat (TPP) pada konsentrasi 0,04% secara signifikan meningkatkan stabilitas nanodispersi serta mempertahankan ukuran partikel pada 131 nm dan nilai indeks polidispersitas (PDI) sebesar 0,265. Evaluasi stabilitas pH, nano-trimetil trisindolin memiliki ukuran partikel rata-rata sebesar 278 nm, PDI 0,324 pada pH 7 dan 289 nm, PDI 0,373 pada pH 5. Evaluasi stabilitas penyimpanan menunjukkan kestabilan yang sangat baik hingga 7 hari dengan ukuran partikel sebesar 136 nm, PDI 0,254. Temuan ini menunjukkan bahwa modifikasi permukaan berbasis gelatin sapi dan cross-linking menggunakan TPP merupakan strategi yang efektif untuk meningkatkan stabilitas nano-trimetil trisindolinuntuk aplikasi antikanker.
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Trimethyl trisindoline is a compound with potential anticancer activity. However, its application is limited by low selectivity. One approach to improve selectivity is the use of nanotechnology-based drug delivery systems, particularly nanodispersions with particle sizes of 10–200 nm. Despite their advantages, nanodispersion systems often suffer from instability due to particle aggregation in biological environments. In this study, a trimethyl trisindoline derivative, 3,3-di(1-methyl-1H-indole-3-yl)-1-methylindolin-2-one, was used as the test compound. The objective of this research was to enhance nanodispersion stability by surface-modifying bovine gelatin. Gelatin was selected for its biocompatibility and biodegradability, as well as its common use in pharmaceutical formulations. Cross-linking with tripolyphosphate was applied to further improve structural stability, and the effect of pH. Nanodispersions were prepared by the reprecipitation method and characterized for particle size using a particle size analyzer and morphology using a field-emission scanning electron microscopy (FESEM). The results showed that bovine gelatin combined with tripolyphosphate (TPP) at specific concentrations significantly improved nanodispersion stability and maintained the size at 131 nm and polydispersity Index (PDI) at 0,265. In the pH stability evaluation, the system exhibited an average particle size of 278 nm with a PDI of 0,324 at pH 7 and 289 nm with a PDI of 0,373 at pH 5. Additionally, the storage stability evaluation showed excellent stability for up to 7 days, with a particle size of 136 nm and a PDI of 0,254. These findings suggest that gelatin-based surface modification with cross-linking is an effective strategy to enhance the stability of nano-trimetyl trisindoline for anticancer applications.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Nano-Obat, Trimetil trisindolin, Modifikasi Permukaan, Cross-linking, Stabilitas, Nano-Drugs, Trimethyl trisindoline, Surface modification, Cross-linking, Stability
Subjects:	Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD547 Flocculation, precipitation, adsorption, etc. Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Dwi Ayu Sekar Wati
Date Deposited:	31 Jul 2026 06:54
Last Modified:	31 Jul 2026 06:54
URI:	http://repository.its.ac.id/id/eprint/140499

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