Fadhila, Aisya Al (2026) Perbandingan Pereduksi Gambir (Uncaria gambir), Hidroksilamin Hidroklorida, dan Asam Format pada Analisa Besi dengan Ion Pengganggu Ca2+ menggunakan Spektrofotometri UV-Vis. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Penelitian ini telah berhasil dilakukan untuk menganalisis kemampuan beberapa agen pereduksi yaitu ekstrak tumbuhan gambir (Uncaria gambir), hidroksilamin hidroklorida, dan asam format dalam mereduksi ion Fe3+ menjadi Fe2+ dengan disertakan ion pengganggu Ca2+ yang kemudian bereaksi dengan 1,10-fenantrolin membentuk kompleks Fe(II)-fenantrolin menggunakan spektrofometer UV-Vis. Penelitian ini mencakup penentuan panjang gelombang maksimum kompleks Fe(II)-fenantrolin, pH optimum, konsentrasi optimum, waktu tunggu yang diperlukan untuk mencapai reaksi optimum, serta pembuatan kurva kalibrasi untuk masing-masing reduktor. Senyawa kompleks Fe(II)-fenantrolin dengan pereduksi gambir, hidroksilamin hidroklorida dan asam format memiliki panjang gelombang pada 510 nm dengan absorbansi yang dihasilkan berturut-turut yaitu 0,315; 0,306 dan 0,201 dengan pH optimum 3,5 ; 5 ; 4,5 dan konsentrasi optimum pada 8 ; 8 ; 12 ppm serta waktu optimum yang diperlukan pada 90 ; 75; 75 menit. Kurva kalibrasi pada gambir menghasilkan r2 sebesar 0,9988, hidroksilamin hidroklorida r2 sebesar 0,9960 dan asam format r2 sebesar 0,9966. Penelitian ini juga membahas sejauh mana gangguan yang ditimbulkan oleh keberadaan ion Ca2+ terhadap analisis kadar Fe, sehingga dapat diketahui efektivitas dan selektivitas masing-masing reduktor yang diuji. Penambahan ion pengganggu Ca2+ dapat dibuktikan dengan penurunan nilai absorbansi pada 3 agen reduktor dengan penambahan konsentrasi ion Ca2+. Ion Ca2+ dengan reduktor gambir mulai mengganggu pada konsentrasi 5 ppm dengan nilai SD 0,001 dan CV 0,375%, sedangkan ion Ca2+ dengan reduktor hidroksilamin hidroklorida mulai mengganggu pada konsentrasi 16 ppm dengan nilai SD 0,001 dan CV 0,408%, serta ion Ca2+ dengan reduktor asam format mulai mengganggu pada konsentrasi 3 ppm dengan nilai SD 0,001 dan CV 0,375%.
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This study was successfully conducted to evaluate the ability of several reducing agents, namely gambir (Uncaria gambir) extract, hydroxylamine hydrochloride, and formic acid, to reduce Fe3+ ions to Fe2+ in the presence of Ca2+ as an interfering ion. The resulting Fe2+ ions subsequently reacted with 1,10-phenanthroline to form the Fe(II)-phenanthroline complex, which was analyzed using UV-Vis spectrophotometry. This research includes determining the maximum wavelength of the Fe(II)-phenanthroline complex, the optimum pH, the optimum concentration, the reaction time required to reach optimum conditions, and constructing calibration curves for each reducing agent. The Fe(II)-phenanthroline complex compounds reduced by gambier, hydroxylamine hydrochloride, and formic acid exhibited wavelengths at 510 nm with resulting absorbances of 0.315 ; 0.306 ; and 0.201, respectively with optimum pH 3,5 ; 5 ; 4,5 and the optimum concentrations were 8 ; 8 ; and 12 ppm, with optimum reaction times of 90 ; 75 ; and 75 minutes, respectively. The calibration curve for gambier yielded an r2 value of 0.9988, hydroxylamine hydrochloride yielded an r2 of 0.9960, and formic acid yielded an r2 of 0.9966. This study also discusses the extent of interference caused by the presence of Ca2+ ions on the analysis of Fe content, thereby determining the effectiveness and selectivity of each tested reducing agent. The interference caused by Ca²⁺ ions can be demonstrated by a decrease in absorbance values for the three reducing agents as the concentration of Ca²⁺ ions increases. Ca²⁺ ions began to interfere with the gambir reducing agent at a concentration of 5 ppm, with an SD of 0.001 and a CV of 0.375%, while Ca²⁺ ions with the hydroxylamine hydrochloride reducing agent began to interfere at a concentration of 16 ppm with an SD of 0.001 and a CV of 0.408%, and Ca²⁺ ions with the formic acid reducing agent began to interfere at a concentration of 3 ppm with an SD of 0.001 and a CV of 0.375%.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Hidroksilamin Hidroklorida; Gambir (Uncaria gambir); Asam Format; Fe(II)-Fenantrolin; Ion Ca2+; Spektrofotometri UV-Vis. ============================================================ Hydroxylamine Hydrochloride; Gambir (Uncaria gambir); Formic Acid; Fe(II)-Phenanthroline; Ca2+ Ion; UV-Vis Spectrophotometry UV-Vis. |
| Subjects: | Q Science > QD Chemistry > QD1 Oxidation-reduction reaction. Q Science > QD Chemistry > QD75.2 Chemistry, Analytic |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Aisya Al Fadhila |
| Date Deposited: | 31 Jul 2026 07:02 |
| Last Modified: | 31 Jul 2026 07:02 |
| URI: | http://repository.its.ac.id/id/eprint/140568 |
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