Perbandingan Metode Analisis Oksitetrasiklin Menggunakan Sensor Elektrokimia Dan Spektrofotometri UV-Vis

Agustin, Chairayni (2026) Perbandingan Metode Analisis Oksitetrasiklin Menggunakan Sensor Elektrokimia Dan Spektrofotometri UV-Vis. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Oksitetrasiklin merupakan antibiotik yang banyak digunakan pada bidang kesehatan, peternakan, dan akuakultur sehingga memerlukan pemantauan residu. Penelitian ini bertujuan membandingkan metode sensor elektrokimia berbasis Screen Printed Carbon Electrode (SPCE) yang dimodifikasi menggunakan Magnetic nanoparticles (MNP) dan Deep Eutectic Solvent (DES) untuk deteksi oksitetrasiklin serta membandingkan kinerjanya dengan metode spektrofotometri UV-Vis. DES dibuat dengan menggunakan asam dekanoat dan 2-pentanol dengan perbandingan molar 1:2. Sensor SPCE dimodifikasi menggunakan suspensi MNP-DES dan dikarakterisasi menggunakan FESEM-EDS. Deteksi oksitetrasiklin dilakukan menggunakan teknik CV dengan variasi pH dan scan rate untuk menentukan kondisi optimum sensor. Kinerja metode pengukuran dievaluasi berdasarkan parameter linearitas, limit deteksi (LOD), limit kuantitasi (LOQ), presisi, dan akurasi. Hasil penelitian menunjukkan bahwa sensor SPCE/MNP-DES bekerja optimum pada larutan PBS pH 4 dengan scan rate 100 mV/s. Sensor menghasilkan hubungan linear pada rentang konsentrasi 1–10 µM dengan koefisien determinasi (R²) sebesar 0,966. Nilai LOD dan LOQ metode elektrokimia masing-masing sebesar 1,438 µM dan 4,358 µM. Metode spektrofotometri UV-Vis menghasilkan nilai R² sebesar 0,997 dengan LOD dan LOQ masing-masing sebesar 0,380 µM dan 1,151 µM. Hasil pengujian presisi menunjukkan nilai RSD sebesar 0,53–1,29% untuk metode elektrokimia dan 0,10–1,90% untuk metode spektrofotometri UV-Vis. Nilai recovery yang diperoleh berturut-turut sebesar 85,98±4,55-90 dan 17±3,02% untuk metode sensor elektrokimia serta 91,39±0,83 dan 136,12±0,24% untuk metode spektrometri UV-Vis. Sensor SPCE/MNP-DES berhasil digunakan untuk deteksi oksitetrasiklin dan menunjukkan kinerja analitik yang baik. Metode elektrokimia menawarkan proses analisis yang lebih sederhana dan cepat, sedangkan metode spektrofotometri UV-Vis memberikan linearitas dan sensitivitas yang lebih tinggi.
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Oxytetracycline is a widely used antibiotic in the fields of healthcare, livestock farming, and aquaculture, making residue monitoring essential. This study aimed to compare an electrochemical sensor based on a Screen Printed Carbon Electrode (SPCE) modified with MNPs and DES for oxytetracycline detection with the UV-Vis spectrophotometric method. The DES was prepared using decanoic acid and 2-pentanol at a molar ratio of 1:2. The SPCE was modified with an MNP-DES suspension and characterized using Field Emission Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (FESEM-EDS). Oxytetracycline detection was performed using cyclic voltammetry (CV) with pH and scan rate variations to determine the optimum sensor conditions. The analytical performance of both methods was evaluated based on linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy. The results showed that the SPCE/MNP-DES sensor exhibited optimum performance in phosphate-buffered saline (PBS) at pH 4 with a scan rate of 100 mV/s. The sensor showed a linear response over the concentration range of 1–10 µM with a coefficient of determination (R²) of 0.966. The LOD and LOQ of the electrochemical method were 1.438 µM and 4.358 µM, respectively. The UV-Vis spectrophotometric method achieved an R² value of 0.997 with LOD and LOQ values of 0.380 µM and 1.151 µM, respectively. The precision test showed relative standard deviation (RSD) values ranging from 0.53–1.29% for the electrochemical method and 0.10–1.90% for the UV-Vis spectrophotometric method. The recovery values obtained 85.98±4.55 and 90.17±3.02% for electrochemical method, 91.39±0,83 and 136.12±0.24% for spectrophotometry UV-Vis method. The SPCE/MNP-DES sensor was successfully applied for oxytetracycline detection and demonstrated good analytical performance. The electrochemical method offered a simpler and faster analytical procedure, whereas the UV-Vis spectrophotometric method provided higher linearity and sensitivity.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	DES, MNP, Oksitetrasiklin, Sensor elektrokimia, SPCE, Teknik CV, CV techniques, DES, Electrochemical sensor, MNPs, Oxytetracycline, SPCE
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD115 Electrochemical analysis
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Analytical Science and Chemical Instrumentation > 47203-(S1) Undergraduate Thesis
Depositing User:	Chairayni Agustin
Date Deposited:	31 Jul 2026 08:40
Last Modified:	31 Jul 2026 08:40
URI:	http://repository.its.ac.id/id/eprint/140593

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