Studi Pengaruh Pelapisan Senyawa Bioaktif pada Silver Nanoparticles (AgNPs) Terhadap Stabilitas dan Aktivitas Antibakteri pada Pengaplikasian Wound Dressing

Sari, Pingky Puspita and Khanza, Nabila Irba (2026) Studi Pengaruh Pelapisan Senyawa Bioaktif pada Silver Nanoparticles (AgNPs) Terhadap Stabilitas dan Aktivitas Antibakteri pada Pengaplikasian Wound Dressing. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Silver nanoparticles (AgNPs) memiliki aktivitas antibakteri yang tinggi, tetapi penggunaannya masih dibatasi oleh kecenderungan aglomerasi dan kestabilan permukaan yang rendah. Penelitian ini bertujuan menganalisis pengaruh pelapisan bromelain dan curcumin terhadap karakteristik AgNPs, aktivitas antibakteri terhadap Staphylococcus aureus, serta kinetika attachment senyawa bioaktif pada permukaan AgNPs. AgNPs dimodifikasi menggunakan bromelain dan curcumin melalui ultrasonikasi selama 0, 10, 20, 30, 40, dan 50 menit. Karakterisasi dilakukan menggunakan FTIR, spektrofotometri UV-Vis, SEM-EDX, kinetika attachment, dan uji antibakteri. Hasil FTIR dan EDX menunjukkan keberadaan gugus fungsi dan unsur organik dari bromelain serta curcumin pada permukaan AgNPs. Analisis UV-Vis menunjukkan bahwa AgNPs–bromelain pada 10 menit mengalami pergeseran puncak SPR menjadi 454,01 nm, sedangkan AgNPs–curcumin pada 30 menit menghasilkan puncak SPR yang lebih jelas pada 429,99 nm dan menunjukkan kestabilan optik relatif lebih baik. Analisis kinetika attachment semu berbasis karbon EDX menunjukkan bahwa AgNPs–curcumin mencapai kadar karbon maksimum pada 20 menit, sedangkan AgNPs–bromelain pada 50 menit. Model Avrami memberikan kecocokan statistik tertinggi dengan nilai R² masing-masing sebesar 0,9967 dan 0,8929. AgNPs tanpa pelapisan menghasilkan zona hambat sebesar 21,75 mm. Aktivitas antibakteri tertinggi diperoleh pada AgNPs–curcumin 50 menit sebesar 33,00 mm, sedangkan AgNPs–bromelain tertinggi pada 30 menit sebesar 18,35 mm. Dengan demikian, pelapisan curcumin lebih efektif meningkatkan aktivitas antibakteri AgNPs, sedangkan bromelain menghasilkan aktivitas yang relatif stabil tetapi belum melampaui AgNPs tanpa pelapisan.
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Silver nanoparticles (AgNPs) exhibit strong antibacterial activity, but their application is limited by agglomeration and low surface stability. This study aimed to analyze the effects of bromelain and curcumin coatings on AgNP characteristics, antibacterial activity against Staphylococcus aureus, and the attachment kinetics of bioactive compounds on the AgNP surface. AgNPs were modified with bromelain and curcumin through ultrasonication for 0, 10, 20, 30, 40, and 50 minutes. Characterization was conducted using FTIR, UV-Vis spectrophotometry, SEM-EDX, kinetic attachment, and antibacterial test. FTIR and EDX results confirmed the presence of functional groups and organic elements originating from bromelain and curcumin on the AgNP surface. UV-Vis analysis showed that AgNPs–bromelain treated for 10 minutes exhibited an SPR peak shift to 454.01 nm, while AgNPs–curcumin treated for 30 minutes produced a clearer SPR peak at 429.99 nm and demonstrated better relative optical stability. The apparent attachment kinetics based on EDX carbon content showed that AgNPs–curcumin reached the maximum carbon content at 20 minutes, whereas AgNPs–bromelain reached the maximum at 50 minutes. The Avrami model provided the best statistical fit, with R² values of 0.9967 and 0.8929, respectively. Uncoated AgNPs produced an inhibition zone of 21.75 mm. The highest antibacterial activity was obtained for AgNPs–curcumin at 50 minutes, with an inhibition zone of 33.00 mm, while AgNPs–bromelain reached its highest value of 18.35 mm at 30 minutes. Therefore, curcumin coating was more effective in enhancing the antibacterial activity of AgNPs, while bromelain coating provided relatively consistent activity but did not exceed that of uncoated AgNPs.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Silver Nanoparticles, Curcumin, Bromelain, Ultrasonic Bath, Antibakteri, Silver nanoparticles, Curcumin, Bromelain, Ultrasonic Bath, Antibacterial
Subjects:	T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Vocational > 24305-Industrial Chemical Engineering Technology
Depositing User:	Pingky Puspita Sari
Date Deposited:	01 Aug 2026 03:45
Last Modified:	01 Aug 2026 03:46
URI:	http://repository.its.ac.id/id/eprint/141279

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