Biodekolorisasi Zat Warna Metilen Biru oleh Isolat Bakteri TGA-7 dari Tambak Garam Osowilangun yang Terimobilisasi pada Matriks SA-PVA-Bentonit

Hapsari, Harnum Prima (2026) Biodekolorisasi Zat Warna Metilen Biru oleh Isolat Bakteri TGA-7 dari Tambak Garam Osowilangun yang Terimobilisasi pada Matriks SA-PVA-Bentonit. Other thesis, Institut Teknologi Sepuluh Nopember.

Text 5004221169-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (4MB) | Request a copy

Abstract

Industri tekstil merupakan salah satu penyumbang utama limbah cair yang mengandung zat pewarna sintetis seperti metilen biru (MB), yang bersifat toksik dan sulit terdegradasi secara alami. Penelitian ini bertujuan untuk mengevaluasi kemampuan biodekolorisasi senyawa MB oleh isolat bakteri TGA-7 yang terimobilisasi dalam matriks SA-PVA-bentonit tanpa penambahan media Zobell Marine Broth (ZMB) pada MB, sebagai kondisi yang menyerupai lingkungan minim nutrien di lapangan. Beads disintesis dengan komposisi SA 1%, PVA 2%, dan bentonit 0,5% (b/v), kemudian digunakan dalam proses dekolorisasi MB 50 mL dengan konsentrasi 50 mg/L selama 7 hari dalam kondisi statis pada suhu 37°C. Hasil penelitian menunjukkan bahwa beads SA-PVA-bentonit-TGA7 memberikan hasil paling baik dengan persentase dekolorisasi 100% yang dicapai pada hari keenam, diikuti oleh SA-PVA-bentonit (91,58%), SA-PVA (36,78%), dan SA (18,01%). Uji reusabilitas menunjukkan bahwa efektivitas dekolorisasi menurun signifikan pada siklus keempat. Scanning hasil dekolor mengalami pergeseran puncak serapan dari 664 nm menjadi 742 nm yang mengindikasikan transformasi struktur kromofor MB oleh aktivitas biologis bakteri TGA-7. Karakterisasi FTIR dan SEM-EDX memperkuat indikasi terjadinya interaksi antara gugus fungsi penyusun beads dengan molekul MB. Penelitian ini membuktikan bahwa imobilisasi bakteri TGA-7 dalam matriks SA-PVA-bentonit efektif untuk aplikasi awal pengolahan limbah pewarna secara biologis dengan kondisi tanpa penambahan media ZMB, walaupun efektivitasnya masih perlu ditingkatkan, misalnya melalui modifikasi struktur matriks dengan penambahan senyawa penstabil.
=====================================================================================================================================
The textile industry is one of the major contributors to wastewater containing synthetic dyes such as methylene blue (MB), which is toxic and resistant to natural degradation. This study aimed to evaluate the biodecolorization capability of MB by the bacterial isolate TGA-7 immobilized in a SA–PVA–bentonite matrix without the addition of Zobell Marine Broth (ZMB) to the MB solution, thereby simulating nutrient-limited environmental conditions. Immobilized beads were prepared using 1% (w/v) SA, 2% (w/v) PVA, and 0.5% (w/v) bentonite, and subsequently applied to the biodecolorization of 50 mL of MB solution at a concentration of 50 mg/L for 7 days under static conditions at 37°C. The results demonstrated that SA–PVA–bentonite beads containing the TGA-7 isolate exhibited the highest biodecolorization efficiency, achieving complete decolorization (100%) on the sixth day. This performance was followed by SA–PVA–bentonite (91.58%), SA–PVA (36.78%), and SA (18.01%). Reusability tests revealed a significant decline in biodecolorization efficiency beginning in the fourth operational cycle. UV–Vis spectral scanning of the decolorized solution showed a shift in the maximum absorption wavelength from 664 nm to 742 nm, indicating the transformation of the MB chromophore structure mediated by the biological activity of the TGA-7 isolate. FTIR and SEM–EDX characterization further confirmed the interaction between the functional groups of the beads and MB molecules. These findings demonstrate that immobilization of the TGA-7 isolate within an SA–PVA–bentonite matrix is effective as a preliminary biological treatment strategy for dye-containing wastewater under conditions without ZMB supplementation. However, further improvements in biodecolorization performance are still required, for example through structural modification of the immobilization matrix by incorporating stabilizing compounds.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi, Metilen Biru, Isolat Bakteri TGA-7, Imobilisasi, SA-PVA-Bentonit, Biodecolorization, Methylene Blue, Bacterial Isolate TGA-7, Immobilization, SA-PVA-Bentonite
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Harnum Prima Hapsari
Date Deposited:	01 Aug 2026 02:23
Last Modified:	01 Aug 2026 02:23
URI:	http://repository.its.ac.id/id/eprint/141549

Actions (login required)

View Item