Nuriyazahra, Salma (2026) Pengaruh Konsentrasi Tembaga-Besi-Asam Galat Bimetalik Metal-Organic Framework dalam Media Kultur terhadap Pertumbuhan Mikroalga Chlorella Sp. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Keterbatasan kelarutan dan perpindahan massa karbon dioksida (CO₂) dalam medium kultur dapat mengurangi ketersediaan karbon anorganik yang dibutuhkan mikroalga untuk fotosintesis. Material metal-organic framework (MOF) berpotensi mendukung retensi dan ketersediaan karbon anorganik dalam medium karena memiliki karakteristik permukaan dan pori yang dapat diakses. Penelitian ini bertujuan mempelajari pengaruh variasi rasio Cu:Fe serta konsentrasi NaOH terhadap yield CuFe-GA MOF serta karakteristiknya dan mengevaluasi pengaruh rasio logam dan konsentrasi penambahan material terhadap pertumbuhan, aktivitas fotosintetik, dan efisiensi fiksasi CO₂ oleh Chlorella sp. CuFe-GA MOF disintesis dalam medium berair pada suhu 30°C menggunakan asam galat sebagai ligan, dengan variasi rasio Cu:Fe sebesar 1:0, 1:1, 1:2, 2:1, dan 0:1 serta variasi konsentrasi NaOH sebesar 0,125; 0,25; 0,5; 1; dan 1,5 M. CuFe-GA MOF dikarakterisasi menggunakan FTIR, XRD, SEM-EDX, dan BET. Selanjutnya, CuFe-GA MOF diaplikasikan ke dalam kultur Chlorella sp. selama 15 hari pada intensitas cahaya 6.500 lux dengan variasi konsentrasi material sebesar 0-20 ppm. Hasil penelitian menunjukkan bahwa konsentrasi NaOH memberikan pengaruh utama yang lebih kuat terhadap yield, sedangkan interaksinya dengan rasio Cu:Fe juga memengaruhi respons yang dihasilkan. Kondisi sintesis bimetalik terpilih diperoleh pada rasio Cu:Fe 1:1 dan NaOH 1 M dengan yield 34,17%, crystallinity index 40,12%, luas permukaan 89,438 m²/g, volume pori 0,1026 cm³/g, dan diameter pori rata-rata 4,5886 nm. Material bimetalik menghasilkan respons pertumbuhan yang lebih baik dibandingkan material monometalik, dengan rasio Cu:Fe 1:1 menunjukkan hasil tertinggi. Penambahan material sebanyak 10 ppm menghasilkan kepadatan sel 1,06 × 10⁸ sel/mL, biomassa 1,7006 g/L, produktivitas biomassa 0,1134 g·L⁻¹·hari⁻¹, klorofil-a 7,105 mg/L, DIC 8,6 mM, dan efisiensi fiksasi CO₂ 24,08%. Peningkatan konsentrasi material menjadi 20 ppm menurunkan pertumbuhan mikroalga. Dengan demikian, CuFe-GA MOF rasio Cu:Fe 1:1 pada konsentrasi 10 ppm merupakan kondisi optimum yang mampu mendukung ketersediaan karbon anorganik, aktivitas fotosintetik, pertumbuhan, dan efisiensi fiksasi CO₂ oleh Chlorella sp.
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The limited solubility and mass transfer of carbon dioxide (CO₂) in the culture medium may reduce the availability of inorganic carbon required by microalgae for photosynthesis. Metal-organic framework (MOF) materials have the potential to support the retention and availability of inorganic carbon in the medium because they possess accessible surface and pore characteristics. This study aimed to investigate the effects of variations in the Cu:Fe ratio and NaOH concentration on the yield and characteristics of CuFe-GA MOF, as well as to evaluate the effects of the metal ratio and material addition concentration on the growth, photosynthetic activity, and CO₂ fixation efficiency of Chlorella sp. CuFe-GA MOF was synthesized in an aqueous medium at 30°C using gallic acid as the ligand, with variations in the Cu:Fe ratios of 1:0, 1:1, 1:2, 2:1, and 0:1, and NaOH concentrations of 0.125, 0.25, 0.5, 1, and 1.5 M. CuFe-GA MOF was characterized using FTIR, XRD, SEM-EDX, and BET. Subsequently, CuFe-GA MOF was applied to Chlorella sp. cultures for 15 days under a light intensity of 6,500 lux, with material concentrations ranging from 0-20 ppm. The results showed that NaOH concentration exerted a stronger main effect on yield, while its interaction with the Cu:Fe ratio also affected the resulting response. The selected bimetallic synthesis condition was obtained at a Cu:Fe ratio of 1:1 and 1 M NaOH, with a yield of 34.17%, a crystallinity index of 40.12%, a surface area of 89.438 m²/g, a pore volume of 0.1026 cm³/g, and an average pore diameter of 4.5886 nm. The bimetallic material produced a better growth response than the monometallic material, with the Cu:Fe ratio of 1:1 showing the highest result. The addition of 10 ppm material resulted in a cell density of 1.06 × 10⁸ cells/mL, biomass of 1.7006 g/L, biomass productivity of 0.1134 g·L⁻¹·day⁻¹, chlorophyll-a of 7.105 mg/L, DIC of 8.6 mM, and a CO₂ fixation efficiency of 24.08%. Increasing the material concentration to 20 ppm reduced microalgal growth. Therefore, CuFe-GA MOF with a Cu:Fe ratio of 1:1 at a concentration of 10 ppm was the optimum condition capable of supporting inorganic carbon availability, photosynthetic activity, growth, and CO₂ fixation efficiency by Chlorella sp.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Chlorella sp., Metal-Organic Framework, Mikroalga, Chlorella sp., Metal-Organic Framework, Microalgae |
| Subjects: | T Technology > TP Chemical technology T Technology > TP Chemical technology > TP1140 Polymers |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering > 24101-(S2) Master Thesis |
| Depositing User: | Salma Nuriyazahra |
| Date Deposited: | 01 Aug 2026 06:58 |
| Last Modified: | 01 Aug 2026 06:58 |
| URI: | http://repository.its.ac.id/id/eprint/141652 |
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