Fernanda, Elfara Ikka (2026) Potensi Endospora Lysinibacillus fusiformis JH2 Dan Bacillus safensis IDN1 Untuk Meningkatkan Kuat Tekan Mortar Semen Yang Mengandung Fly Ash. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Penggunaan fly ash untuk substitusi sebagian semen dapat mengurangi penggunaan semen konvensional, tetapi substitusi dalam jumlah tinggi dapat menurunkan kekuatan awal mortar. Penambahan endospora bakteri berpotensi meningkatkan performa mortar melalui aktivitas biomineralisasi. Penelitian ini bertujuan menganalisis pengaruh penambahan endospora Lysinibacillus fusiformis JH2 dan Bacillus safensis IDN1 terhadap sifat mekanik, porositas, dan viabilitas endospora pada mortar semen yang mengandung fly ash. Penelitian menggunakan mortar semen dengan dan tanpa substitusi 50% fly ash kelas F serta penambahan endospora kedua jenis bakteri. Pengujian meliputi kuat tekan, kuat tarik belah, dan porositas mortar pada umur 7 dan 28 hari serta viabilitas endospora dalam mortar. Hasil penelitian menunjukkan bahwa kuat tekan seluruh perlakuan meningkat seiring bertambahnya umur mortar, dengan nilai 19,49–38,27 MPa pada umur 28 hari. Penambahan endospora pada mortar yang mengandung fly ash menghasilkan kuat tekan lebih tinggi dibandingkan mortar fly ash tanpa endospora, dengan nilai tertinggi sebesar 36,09 MPa pada perlakuan dengan B.safensis IDN1. Analisis statistik menunjukkan bahwa substitusi fly ash, jenis bakteri, dan interaksi keduanya berpengaruh nyata terhadap kuat tekan mortar umur 28 hari (p<0,05). Penambahan endospora menghasilkan respons yang berbeda terhadap kuat tarik belah dan porositas mortar. Endospora masih dapat ditemukan dalam mortar setelah proses perawatan yang ditunjukkan dengan viabilitas pada media Nutrient Agar. Secara keseluruhan, Bacillus safensis IDN1 menunjukkan potensi yang lebih baik dibandingkan Lysinibacillus fusiformis JH2 berdasarkan peningkatan kuat tekan dan viabilitas endospora pada mortar yang mengandung fly ash.
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Fly ash can be used as a partial cement substitute to reduce conventional cement consumption, although high substitution levels may decrease the early strength of mortar. This study aimed to analyze the effects of Lysinibacillus fusiformis JH2 and Bacillus safensis IDN1 endospores on the mechanical properties, porosity, and endospore viability of cement mortar containing fly ash. Mortar was prepared with and without 50% Class F fly ash substitution and bacterial endospore addition. Compressive strength, splitting tensile strength, and porosity were evaluated at 7 and 28 days, along with endospore viability. The results showed that compressive strength increased with mortar age, ranging from 19.49 to 38.27 MPa at 28 days. Endospore addition increased the compressive strength of fly ash-containing mortar compared with fly ash mortar without endospores. The highest compressive strength within this group was 36.09 MPa in the MFE treatment containing B. safensis IDN1. Fly ash substitution, bacterial species, and their interaction significantly affected 28-day compressive strength (p<0.05). Endospore addition produced different responses in the splitting tensile strength and porosity depending on the bacterial species and mortar composition. Endospores remained viable after the curing period, as confirmed by their growth on Nutrient Agar medium. Overall, Bacillus safensis IDN1 demonstrated better performance than Lysinibacillus fusiformis JH2 in terms of improving compressive strength and maintaining endospore viability in fly ash-containing mortar.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Bacillus safensis IDN1, endospores, fly ash, compressive strength, Lysinibacillus fusiformis JH2. ======================================================================================================================== Bacillus safensis IDN1, endospora, fly ash, kuat tekan, Lysinibacillus fusiformis JH2. |
| Subjects: | Q Science Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Elfara Ikka Fernanda |
| Date Deposited: | 01 Aug 2026 04:56 |
| Last Modified: | 01 Aug 2026 04:56 |
| URI: | http://repository.its.ac.id/id/eprint/141660 |
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