Bariroh, Riris Dwi Indah (2019) Pengaruh Variasi Pemberian Aerasi dan Pupuk Diatom Terhadap Pertumbuhan dan Kandungan Lipid Mikroalga Skeletonema Costatum. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Skeletonema costatum adalah mikroalga diatom dengan kandungan lipid sebesar 7,42%. Pertumbuhan S. costatum dipengaruhi beberapa faktor salah satunya yaitu aerasi. Aerasi berfungsi untuk sirkulasi air pada media kultur, sehingga tidak terjadi proses startifikasi. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan aerasi dengan kombinasi pupuk diatom pada pertumbuhan dan kandungan lipid S. costatum. Parameter yang diamati meliputi perhitungan kepadatan sel, biomassa dan uji TAG menggunakan mikroskop fluorescence serta total lipid dengan metode Soxhletasi. Hasil penelitian menunjukkan bahwa perlakuan P1(komposisi pupuk KNO3 75 gr/l dan Na2SiO3 30 gr/l) dengan aerasi 12 jam berpengaruh terhadap kepadatan sel S. costatum dengan jumlah 395 x 103 sel/ml. Perlakuan P3 (komposisi pupuk KNO3 37,5 gr/l dan Na2SiO3 15 gr/l) dengan aerasi 36 jam berpengaruh terhadap biomassa S. costatum sebesar 0,40 gram. Perlakuan P2 (komposisi pupuk KNO3 0 gr/l dan Na2SiO3 15 gr/l) dengan cekaman aerasi 12, 24 menunjukkan kandungan TAG dengan pendaran warna kuning serta kandungan total lipid sebesar 70%.
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Skeletonema costatum is microalgae diatom with lipid content of 7.42%. The growth of S. costatum is influenced by several factors, one of them is aeration. Aeration function is to circulate the water on culture media so that the startification process does not occur. This study aim is to determine the effect of aeration treatment with a combination of diatom fertilizer on the growth and lipid content of S. costatum. S. costatum is treated then calculated cell density, biomass and TAG test using fluorescence microscopy and total lipids using the Soxhletation method. The results show that P1 (KNO3 75 gr / l and Na2SiO3 30 gr / l) with an aeration of 12 hours has an effect on the density of S. costatum cells in the amount of 395 x 103 cells/ml. The treatment of P3 (KNO3 37.5 gr / l and Na2SiO3 15 gr / l) with aeration of 36 hours affected the biomass of S. costatum by 0.40 grams. The treatment of P2 (KNO3 0 gr / l and Na2SiO3 15 gr / l) with aeration stresses 12, 24 hours show TAG content with yellow emission and total lipid content 70%.
Item Type: | Thesis (Other) |
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Additional Information: | RSBi 572.57 Bar p-1 2019 |
Uncontrolled Keywords: | aerasi, lipid,mikroalga, S. costatum. |
Subjects: | Q Science > QK Botany > QK568 Microalgae |
Divisions: | Faculty of Natural Science > Biology > 46201-(S1) Undergraduate Thesis |
Depositing User: | Riris Dwi Indah Bariroh |
Date Deposited: | 02 Jan 2024 07:18 |
Last Modified: | 02 Jan 2024 07:18 |
URI: | http://repository.its.ac.id/id/eprint/64108 |
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