Pengaruh Fitohormon IAA dan BAP pada Kultur Nannochloropsis sp. Terhadap Biomassa, Laju Pertumbuhan dan Komposisi Asam Lemak

Kurniawan, Hendra Bayu (2019) Pengaruh Fitohormon IAA dan BAP pada Kultur Nannochloropsis sp. Terhadap Biomassa, Laju Pertumbuhan dan Komposisi Asam Lemak. Other thesis, Institut Teknologi Sepuluh Nopember (ITS).

Text 01311440000052-Undergradutes_Thesis.pdf Restricted to Repository staff only Download (4MB) | Request a copy

Abstract

Salah satu jenis mikroalga yang dibudidayakan sebagai kandidat biodiesel adalah Nannochloropsis sp. Pertumbuhan populasi Nannochloropsis sp. dapat berlangsung secara terus-menerus tergantung pada hasil asimilasi, hormon dan zat pengatur tumbuh, serta lingkungan yang mendukung. Fitohormon memainkan peran penting sebagai pengatur pertumbuhan dan pembelahan sel pada mikroalga. Penelitian ini bertujuan untuk mengetahui pengaruh IAA (auksin) dan BAP (sitokinin) terhadap laju pertumbuhan, biomassa, kandungan triasilgliserol (TAG) secara kualitatif, dan komposisi asam lemak pada Nannochloropsis sp. Pengamatan TAG secara kualitatif dilakukan berdasarkan pendaran warna yang dihasilkan dari pewarnaan Nile red di bawah mikroskop fluoresensi. Parameter yang diamati adalah laju pertumbuhan, biomassa, analisis kualitatif TAG, dan analisis komposisi asam lemak. Hasil analisis data menunjukkan bahwa penambahan variasi fitohormon IAA dan BAP berpengaruh terhadap laju pertumbuhan mikroalga, di mana laju pertumbuhan mikroalga pada perlakuan kombinasi fitohormon P15 tidak berbeda signifikan dengan perlakuan kombinasi fitohormon P13, perlakuan kombinasi fitohormon P14, dan kombinasi fitohormon P16. Sedangkan perlakuan kombinasi fitohormon P1 (kontrol) tidak berbeda signifikan dengan perlakuan kombinasi fitohormon P2. Pada semua perlakuan kombinasi fitohormon IAA dan BAP terdapat pendaran warna merah yang mengindikasikan adanya endapan TAG yang rendah. Analisis profil asam lemak pada perlakuan kombinasi fitohormon P8 mengandung 5 jenis asam lemak, sedangkan perlakuan kombinasi fitohormon P1 hanya mengandung 4 jenis asam lemak. Asam lemak dokosanoat muncul pada perlakuan kombinasi fitohormon P16 tetapi tidak ditemukan pada perlakuan kombinasi fitohormon P1. Asam lemak palmitat pada perlakuan kombinasi fitohormon P16 ppm memiliki kandungan lebih tinggi yaitu sebesar 45,55% sehingga cocok untuk produksi biodiesel.
===================================================================================================================================
One type of microalgae that is cultivated as a candidate for biodiesel is Nannochloropsis sp. The growth of the population of Nannochloropsis sp can take place continuously depending on the results of assimilation, hormones and growth substances and the supporting environment. Phytohormone plays an important role as a growth regulator and cell division in microalgae. This study aims to determine the effect of IAA (auxin) and BAP (cytokinin) on growth rate, biomass, triacylglycerol (TAG) content qualitatively and fatty acid composition in Nannochloropsis sp. Qualitative TAG observation was carried out based on color glow produced from Nile red staining under a fluorescent microscope. The parameters observed were growth rate, biomass, qualitative analysis of TAG and analysis of fatty acid composition. The results of data analysis showed that the addition of variations in IAA and BAP phytohormones had an effect on the growth rate of microalgae where the growth rate of microalgae in the treatment of phytohormone P15 combination was not significantly different from the combination treatment of phytohormone P13, combination treatment of phytohormone P14, combination of phytohormone P16. While the combination treatment of phytohormone P1 (control) was not significantly different from the combination treatment of phytohormone P2. In all IAA and BAP combination phytohormone treatments there was a red glow which indicated the presence of low TAG deposits. Analysis of fatty acid profiles in the combination treatment of phytohormone P8 contained 5 kinds of fatty acids while the combination treatment of phytohormone P1 contained only 4 kinds of fatty acids. Docosanoic fatty acids appear in the combination treatment of phytohormone P16 but were not found in the combination treatment of phytohormone P1. Palmitic fatty acids in the treatment of the combination of phytohormone P16 ppm had more content of 45.55% making it suitable for biodiesel production.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Auxin, BAP, Cytokinin, Nannochloropsis sp. , IAA, Composition of Fatty Acids, and tags (triacylglycerol).
Subjects:	Q Science > QH Biology Q Science > QK Botany Q Science > QK Botany > QK568 Microalgae
Divisions:	Faculty of Natural Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Kurniawan Hendra Bayu
Date Deposited:	20 Jul 2026 06:53
Last Modified:	20 Jul 2026 06:53
URI:	http://repository.its.ac.id/id/eprint/69573

Actions (login required)

View Item