Sari, Dega Nurita (2015) Pengaruh Penambahan Bakteri Ralstonia Pickettii Terhadap Biodegradasi Ddt Oleh Jamur Pelapuk Putih Pleurotus Eryngii. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Pengaruh penambahan bakteri Ralstonia pickettii
terhadap biodegradasi DDT oleh jamur pelapuk putih
Pleurotus eryngii telah diteliti. R. pickettii ditambahkan ke
dalam 10 mL kultur P. eryngii masing-masing sebanyak 1, 3,
5, 7 dan 10 ml (1 ml ≈ 1,337 x 109 sel). Bakteri R. pickettii
menghasilkan biosurfaktan untuk meningkatkan kelarutan
DDT sehingga DDT dapat lebih mudah terdegradasi. Analisis
degradasi DDT menggunakan HPLC dan Kromatografi GCMS.
Degradasi DDT tertinggi ditunjukkan pada penambahan
7 mL bakteri R. pickettii dengan jumlah degradasi sebesar
78,23 % sedangkan jumlah degradasi DDT terendah terjadi
pada penambahan bakteri 5 ml dengan jumlah degradasi
sebesar 36,17 %. Metabolit produk yang dihasilkan adalah
DDE [1,1-dichloro-2,2-bis(p-chlorophenyl)ethyene] dan
DDD [1,1-dichloro-2,2-bis(p-chlorophenyl)ethane].
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Effect of addition of bacteria Ralstonia pickettii on
biodegradation of DDT by white-rot fungus Pleurotus eryngii
had been investigated. R. pickettii was added into 10 mL P.
eryngii culture at 1, 3, 5, 7 and 10 ml (1 ml ≈ 1,337 x 109
cells). R. picketti bacteria produced biosurfactant to increased
solubility DDT so that DDT could be eassier degraded. DDT
degradation analysis used HPLC and Chromatography GCMS.
The addition of 7 ml of R. pickettii showed the highest
DDT degradation about 78,23 %, while the addition of 5 mL
of R. picketii showed the lowest one about 36,17 %. The type
of metabolic product formed is DDE [1,1-dichloro-2,2-bis(pchlorophenyl)
ethyene] and DDD [1,1-dichloro-2,2-bis(pchlorophenyl)
ethane].
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSKi 579.332 Sar p |
| Uncontrolled Keywords: | Biodegradasi, Pleurotus eryngii, Ralstonia pickettii, DDT, HPLC, Kromatografi GC-MS |
| Subjects: | Q Science > QD Chemistry > QD117 Absorption |
| Divisions: | Faculty of Mathematics and Science > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 18 Nov 2019 11:05 |
| Last Modified: | 18 Nov 2019 11:05 |
| URI: | http://repository.its.ac.id/id/eprint/71866 |
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