., Fajriah (2016) Pengaruh Penambahan Bakteri Terhadap Degradasi DDT Oleh Phlebiabrevispora. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) adalah polutan
organik yang persisten di alam dan memiliki dampak buruk bagi lingkungan,
binatang dan manusia. Phlebia brevispora memiliki kemampuan dalam
mendegradasi DDT, akan tetapi masih tergolong rendah dan memerlukan waktu
degradasi yang lama. Dalam penelitian ini, pengaruh penambahan bakteri
(Pseudomonas aeruginosa, Bacilllus subtilis, dan Ralstonia pickettii) pada
biodegradasi DDT oleh P. brevispora telah diteliti, dimana masing-masing bakteri
ditambahkan sebanyak 1, 3, 5, 7 dan 10 mL ke dalam kultur P. brevispora.
Degradasi DDT oleh P. brevispora tanpa penambahan bakteri sebesar 64%.
Penambahan 10 mL P. aeruginosa mampu meningkatkan degradasi DDT oleh P.
brevispora dengan sempurna (100%). 1,1-dikloro-2,2-bis (4-klorofenil) etana
(DDD) dan 1-kloro-2,2-bis (4-klorofenil) etilen (DDMU) merupakan metabolit
produk dari DDT oleh kultur campuran P. brevispora dan P. aeruginosa. Reduktif
deklorinasi merupakan reaksi tahap pertama pada proses biodegradasi DDT oleh
P.brevispora dengan penambahan P. aeruginosa.
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1,1,1-Trichloro -2,2-bis(4-chlorophenyl)ethane (DDT) is an organic pollutant that
persisten in nature and has bad effect for the environment, animal and human.
Phlebia brevispora has an ability to degrade DDT, but still relatively low and
need longer time. In this study, the effect of adding bacteria (Pseudomonas
aeruginosa, Bacillus subtilis, and Ralstonia pickettii) on biodegradation of DDT
by P. brevispora was investigated, which each bacterium was added by 1, 3, 5, 7
and mL into 10 mL P. brevispora culture. Degradation of DDT by P. brevispora
without addition of bacteria was 64%. However, the addition of P. aeruginosa
was able to increase the degradation of DDT by P.brevispora completely (100%).
1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) and 1-chloro-2,2-bis(4-
chlorophenyl)ethylene (DDMU) were metabolite product of DDT. Reductive
dechlorination was the first step reaction on DDT biodegradation process by P.
brevispora with the addition of P. aeruginosa.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | RTKi 579.332 Faj p |
| Uncontrolled Keywords: | Biodegradasi, DDT, Phlebia brevispora, Bacillus subtilis |
| Subjects: | Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry |
| Divisions: | Faculty of Mathematics and Science > Chemistry > 47101-(S2) Master Thesis |
| Depositing User: | ansi aflacha |
| Date Deposited: | 27 Jul 2020 02:14 |
| Last Modified: | 27 Jul 2020 02:14 |
| URI: | http://repository.its.ac.id/id/eprint/76532 |
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