Aktivitas Trisindolina-1 Memicu Autophagy-like Cell Death Dan Meningkatkan Sensitivitas Cancer Stem Cell Terhadap Doxorubicin

Dwi Ersandy, Alfani Raziful (2021) Aktivitas Trisindolina-1 Memicu Autophagy-like Cell Death Dan Meningkatkan Sensitivitas Cancer Stem Cell Terhadap Doxorubicin. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Cancer stem cells merupakan subpopulasi kanker yang mampu memperbarui dan memperbanyak sel dengan cepat. CSC juga diyakini sebagai penyebab resistensi obat dan kekambuhan tumor. Trisindolina-1 diketahui bersifat anti-kanker pada berbagai sel lini, namun efeknya terhadap CSC sampai saat ini masih belum diketahui. Penelitian bertujuan untuk mengetahui aktivitas antikanker trisindolina-1 (TS1) serta efeknya saat dikombinasikan dengan doxorubicin (DOX) pada CSC. MTT assay digunakan untuk mengetahui proliferasi CSC setelah perlakuan selama 24 jam. Annexin-V/PI staining dan analisis flowcytometry digunakan untuk mengevaluasi apoptosis. Hasil penelitian menunjukkan bahwa TS1 menghambat proliferasi CSC pada konsentrasi tinggi (IC50 91,11 µM) dan sel menampilkan karakter mirip autofagi, yaitu adanya vesikel intraseluler. DOX menghambat proliferasi CSC pada konsentrasi yang lebih rendah dibandingkan TS1 (IC50 19.85 µM). Perlakuan kombinasi antara TS1 dan DOX menunjukkan efek sinergisme serta meningkatkan terjadinya apoptosis secara signifikan dibandingkan perlakuan tunggal. Dari hasil ini, disimpulkan bahwa TS1 memiliki potensi sebagai antikanker. Selain itu, kombinasi TS1 dan DOX dapat menjadi agen terapeutik yang efektif melawan CSC.
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Cancer stem cells are defined as a subpopulations that are able to self-renewal and multiply rapidly. CSC is also believed to be a cause of drug resistance and tumor recurrence. Trisindolina1 is known to be anti-cancer in various cell lines, but its effect on CSC is still unknown. The aim of the study was to determine the anti-cancer activity of trisindoline-1 (TS1) and its effect when combined with doxorubicin (DOX) on CSC. MTT assay was used to determine CSC proliferation after 24 hours of treatment. Annexin-V/PI staining and flowcytometry analysis were used to evaluate apoptosis. The results showed that TS1 inhibited CSC proliferation at high concentrations (IC50 91.11 µM) and the cells displayed autophagy-like characteristics, namely the presence of intracellular vesicles. DOX inhibited CSC proliferation at a lower concentration than TS1 (IC50 19.85 µM). The combination treatment between TS1 and DOX showed a synergistic effect and significantly increased the occurrence of apoptosis compared to the single treatment. From these results, it was concluded that TS1 has potential as an anticancer. In addition, the combination of TS1 and DOX can be an effective therapeutic agent against CSC.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Apoptosis, Autofagi, CSC, Sinergisme, Trisindolina-1 Apoptosis, Autophagy, CSC, Synergism, Trisindoline-1
Subjects:	Q Science > QP Physiology > QP624 Molecular biology.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Alfani Raziful Dwi Ersandy
Date Deposited:	26 Jul 2021 23:49
Last Modified:	26 Jul 2021 23:52
URI:	http://repository.its.ac.id/id/eprint/84495

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