Biodekolorisasi Pewarna Malachite Green oleh Bakteri Pseudomonas aeruginosa

Rahmasari, Riski Amalia (2021) Biodekolorisasi Pewarna Malachite Green oleh Bakteri Pseudomonas aeruginosa. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Malachite green (MG) merupakan pewarna trifenilmetana yang digunakan untuk memberi warna berbagai bahan seperti kapas, kayu, sutra, kulit, dan kertas. MG memiliki sifat persisten terhadap lingkungan dan sangat beracun bagi berbagai hewan air dan darat. Pada penelitian ini, dilakukan penghilangan warna pada MG dengan menggunakan bakteri Pseudomonas aeruginosa pada media cair Luria-Bertani Broth (LB). Analisis dilakukan dengan menggunakan instrumen UV-Vis. Dari hasil penelitian diperoleh optimalisasi pada proses dekolorisasi pewarna MG dengan bakteri P. aeruginosa pada konsentrasi MG 50 mg/L dengan waktu inkubasi 24 jam pada suhu 40 °C dengan pH 7 pada kondisi didiamkan. Hasil menunjukkan bahwa P. aeruginosa dengan kondisi optimum memiliki kemampuan dalam mendekolorisasi pewarna MG sebesar 96,5 %.
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Malachite green (MG) is a triphenylmethane dye that is used to color various materials such as cotton, wood, silk, leather, and paper. MG is environmentally persistent and highly toxic to a variety of aquatic and terrestrial animals. In this study, the decolorization of MG was investigated using bacterium Pseudomonas aeruginosa in Luria-Bertani broth media. Decolorization of MG was analyzed using UV-Vis spectrophotometer. The results showed that optimization of the decolorization process of MG dye with bacterium P. aeruginosa was obtained at a MG concentration of 50 mg/L with an incubation time of 24 hours, a temperature of 40 °C with a pH of 7 under static conditions. The results showed that P. aeruginosa with its optimum conditions had the ability to decolorize MG dyes approximately 96,5 %.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Malachite Green, Pseudomonas aeruginosa, Biodegradasi, Biodekolorisasi. Malachite Green, Biodegradation, Biodecolorization.
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry T Technology > TD Environmental technology. Sanitary engineering > TD192.5 Bioremediation
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Riski Amalia Rahmasari
Date Deposited:	23 Aug 2021 15:35
Last Modified:	23 Aug 2021 15:35
URI:	http://repository.its.ac.id/id/eprint/89144

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