Sintesis Karbon Sphere Dari Ampas Tebu Sebagai Material Antibakteri Menggunakan Metode Microwave-Assisted Synthesis

Mumtaz, Muhammad Fikri (2022) Sintesis Karbon Sphere Dari Ampas Tebu Sebagai Material Antibakteri Menggunakan Metode Microwave-Assisted Synthesis. Other thesis, Institut Teknologi Sepuluh Nopember Surabaya.

Text 02311840000115-Undergraduate_Thesis_Muhammad Fikri Mumtaz.pdf - Accepted Version Restricted to Repository staff only until 1 September 2024. Download (5MB) | Request a copy

Abstract

Resistensi antibiotik merupakan topik yang menjadi perhatian saat ini karena banyaknya bakteri patogen yang telah menjadi resisten terhadap agen antibiotik. Hal ini disebabkan karena bakteri berubah dari waktu ke waktu dan tidak lagi merespon obat-obatan. Oleh karena itu, material antibakteri terus dikembangkan termasuk pemanfaatan nanomaterial. Carbon Sphere (CS) merupakan nanomaterial yang mempunyai aktivitas antibakteri yang dapat menghambat bakteri dan membunuh bakteri. Dalam penelitian ini, ampas tebu disintesis menjadi CS menggunakan metode Microwave-Assisted Synthesis dengan variasi pelarut air, etilen glikol, dan dietilen glikol serta variasi daya sebesar 240, 400, dan 800 W. Karakterisasi fisikokimia CS dilakukan menggunakan Scanning Electronic Microscope, Spektroskopi Ultraviolet-visible, Fourier Transform Infra-Red, dan Dynamic Light Scattering. Aktivitas antibakteri CS dievaluasi dengan uji difusi cakram Kirby-Bauer untuk mengetahui konsentrasi hambat minimum (minimum inhibitory concentration, MIC) pada kondisi cahaya tampak dan kondisi gelap terhadap bakteri E. coli (Gram positif) dan S. aureus (Gram negatif). Hasil aktivitas antibakteri terbaik ditunjukkan pada kondisi terang dengan CS disintesis dengan etilen glikol dan dietilen glikol pada daya 240 W dengan MIC 1 mg/mL terhadap E. coli dan S. aureus dengan CS variasi air berdaya 400 W bernilai MIC 1 mg/mL. Pada kondisi gelap, CS yang disintesis dengan pelarut dietilen glikol (240 W), air dan etilen glikol (800 W) dihasilkan MIC sebesar 1 mg/mL terhadap E. coli dan dihasilkan MIC 8 mg/mL terhadap S. aureus untuk CS yang disintesis dengan pelarut air 400 W
==========================================================================================================================================
Antibiotic resistance constitutes a recent topic of concern since many pathogenic bacteria have become resistant to antibiotic agents. This is because bacteria change over time and no longer respond to drugs. Therefore, antibacterial materials continue to be developed, including the use of nanomaterials. Carbon Sphere (CS) is a nanomaterial that has antibacterial activity that can inhibit as well as kill bacteria. In this study, bagasse was synthesized into CS using the Microwave-Assisted Synthesis with variations in solvents, including water, ethylene glycol, and diethylene glycol, and variations in microwave power of 240, 400, and 800 W. Physicochemical characterization of CS was carried out using Scanning Electronic Microscope, Ultraviolet-visible Spectroscopy, Fourier Transform Infra-Red, and Dynamic Light Scattering. The antibacterial activity of CS was evaluated by the Kirby-Bauer disc diffusion test to determine the minimum inhibitory concentration (MIC) in visible light and dark conditions against E. coli (Gram positive) and S. aureus (Gram negative) bacteria. The best antibacterial activities were shown under bright conditions with CS synthesized using ethylene glycol and diethylene glycol at a power of 240 W with a MIC of 1 mg/mL against E. coli while CS variation of water with a MIC of 1 mg/mL against S. aureus. Under dark conditions, CS synthesized with diethylene glycol (240 W), water and ethylene glycol (800 W) resulted in a MIC of 1 mg/mL against E. coli and an MIC of 8 mg/mL against S. aureus is obtained for CS synthesized using water at a power of 400 W

Item Type:	Thesis (Other)
Additional Information:	RSF 620.5 Mum s-1
Uncontrolled Keywords:	MIC, Antibakteri, Material Nano, Microwave, Karbon
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA455 Carbon. Nanotubes.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Physics Engineering > 30201-(S1) Undergraduate Thesis
Depositing User:	EKO BUDI RAHARJO
Date Deposited:	10 Jan 2023 01:20
Last Modified:	10 Jan 2023 01:20
URI:	http://repository.its.ac.id/id/eprint/95349

Actions (login required)

View Item