Sintesis Karbon Dots Terdoping N dan S dari Limbah Ampas Teh sebagai Material Antibakteri dan Deteksi Dopamin

Putra, Febryan Nugraha (2023) Sintesis Karbon Dots Terdoping N dan S dari Limbah Ampas Teh sebagai Material Antibakteri dan Deteksi Dopamin. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Gangguan kesehatan fisik dapat disebabkan oleh infeksi bakteri, serta gangguan psikis pada manusia. Bakteri dapat membentuk biofilm yang mampu melindungi bakteri dari antibiotik, sehingga menjadi permasalahan untuk penyakit yang menular. Hal tersebut menyebabkan diperlukan material antibakteri alternatif. Selain itu, skizofrenia sebagai salah satu gangguan kejiwaan dapat mengganggu fungsi kognitif manusia, yang salah satu indikasinya adalah kenaikan dopamin. Carbon dots dikembangkan untuk dapat menjadi material antibakteri dan sensor dopamin. Penggunaan nitrogen dan sulfur sebagai material doping CDs mampu meningkatkan performa CDs sebagai material antibakteri dan sensor dopamin. Pengembangan CDs terdoping nitrogen dan sulfur dilakukan dengan variasi rasio (C:N) 1:3, 1:5, 1:7 gr/ml, pH 6, 7, dan 8, serta bersumber dari ampas teh hitam dan teh hijau. Karakterisasi dilakukan dengan menggunakan UV-Vis dan Fluorescence spektrometer, Fourier Transform Infrared (FTIR), dan Dynamic Ligth Scattering (DLS). Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi cair untuk menentukan efektivitas aktivitas antibakteri terhadap bakteri E. coli (Gram negatif) dan S. aureus (Gram positif) berdasarkan nilai Optical Density (OD) terendah. Hasil yang didapatkan diantaranya, partikel yang berukuran terkecil adalah N,S-CDs dengan variasi pH 6 dengan permukaan negatif. Nilai OD terendah adalah 0.076 dari kontrol 0.719 pada bakteri E. coli, dan 0.129 dari kontrol 0.394 pada bakteri S. aureus. Deteksi dopamin memiliki nilai LOD 99 μM dan 301 μM LOQ.
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Physical health problems can be caused by bacterial infections, as well as psychological disorders in humans. Bacteria can form biofilms that can protect itself from antibiotics, and will be dangerous for disease than can’t be treated. This causes the need for alternative antibacterial materials. In addition, schizophrenia as a psychiatric disorder can interfere with human cognitive function, one indication of which is an increase in dopamine. Carbon dots were developed as antibacterial materials and dopamine sensors. The use of nitrogen and sulfur as CDs doping materials can improve the performance of CDs as antibacterial materials and dopamine sensors. Development of CDs doped with nitrogen and sulfur was carried out with various ratios (C:N) 1:3, 1:5, 1:7 g/ml, pH 6, 7 and 8, and sourced from black tea and green tea dregs. Characterization was carried out using UV-Vis and Fluorescence spectrometers, Fourier Transform Infrared (FTIR), and Dynamic Light Scattering (DLS). Antibacterial activity test was carried out using the liquid diffusion method to determine the effectiveness of antibacterial activity against E. coli (Gram negative) and S. aureus (Gram positive) bacteria based on the lowest Optical Density (OD) value. Among the results obtained, the smallest sized particles were N,S-CDs with a variation of pH 6 with a negative surface. The lowest OD (Optical Density) value was 0.076 out of control 0.719 on E. coli bacteria, and 0.129 out of control 0.394 on S. aureus bacteria. Dopamine detection has a LOD (Limit of Detection) value of 99 μM and 301 μM LOQ (Limit of Quantitation).

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Antibakteri, Carbon Dots, Dopamin, Optical Density, Antibacterial, Dopamine
Subjects:	Q Science Q Science > QC Physics Q Science > QC Physics > QC451 Spectroscopy Q Science > QC Physics > QC475 Photoluminescence T Technology > TA Engineering (General). Civil engineering (General) > TA455 Carbon. Nanotubes.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Physics Engineering > 30201-(S1) Undergraduate Thesis
Depositing User:	Febryan Nugraha Putra
Date Deposited:	20 Jul 2023 06:59
Last Modified:	20 Jul 2023 06:59
URI:	http://repository.its.ac.id/id/eprint/98709

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