Azzamzami, Muhammad Aditya (2023) Analisis Pengaruh Rasio Collagen/Alginate dan Penambahan β-Tricalcium Phosphate terhadap Sifat Mekanik dan Bioaktivitas Scaffold Komposit Hidrogel pada Rekonstruksi Mikrotia. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Mikrotia merupakan sebuah kelainan berupa kurang lengkapnya struktural daun telinga hingga tidak ada sama sekali. Secara keseluruhan, kasus mikrotia sekitar 1-4 per 10.000 kelahiran dengan 10% mengalami mikrotia di kedua telinga. Perbaikan dan penggantian jaringan untuk penanganan mikrotia dapat dilakukan dengan dua metode, yaitu menggunakan tulang rawan rusuk autogenous dan implan sintetis. Namun, dalam metode-metode tersebut dapat menyebabkan komplikasi pada area donor dan tingginya tingkat infeksi. Berdasarkan hal tersebut, komposit hidrogel berbahan collagen, β-TCP, dan alginate menjadi salah satu pilihan untuk dapat dijadikan sebagai scaffold dalam penanganan kasus mikrotia. Collagen mempunyai sifat biokompatibilitas dan biodegradasi yang mana dapat dikombinasikan dengan alginate,. Hal tersebut dikarenakan alginate dapat menjadi matriks pendukung. Sementara β-TCP ditambahkan untuk meningkatkan sifat mekanik. Tujuan dari penelitian adalah menganalisis pengaruh rasio collagen/alginate sebesar 3:1, 2:1, dan 1:1 serta penambahan β-TCP sebesar 5, 10, dan 15% wt dalam scaffold komposit hidrogel terhadap morfologi (SEM-EDX), sifat mekanik (uji tekan), sifat fisik (uji swelling), dan biokompatibilitas (uji MTT assay) untuk kasus rekonstruksi mikrotia. Hasil SEM menunjukkan terdapat porositas pada setiap variasi, dengan variasi rasio collagen dan alginate yang paling besar terdapat pada rasio 3:1 dengan penambahan β-TCP 10%. Sementara untuk variasi perubahan persentase β-TCP terdapat pada variasi β-TCP 15% sebesar 16,66%. Pada uji tekan didapatkan hasil nilai modulus elastisitas dengan nilai modulus elastisitas tertinggi pada variasi rasio collagen dan alginate 1:1 dengan penambahan β-TCP 5% sebesar 1,676 ± 0,27 MPa. Nilai maksimum swelling dengan variasi penambahan persentase β-TCP didapatkan kisaran swelling ratio paling besar adalah 249,25 ± 6,91 % pada persentase β-TCP 5% dengan variasi waktu 90 menit. Sementara pada variasi rasio perubahan collagen dan alginate pada β-TCP 10% dihasilkan swelling ratio sebesar 237,14 ± 10,18 % pada rasio 1:1 dengan variasi waktu 90 menit.
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Microtia is a disorder in the form of structural incompleteness of the auricle to the point where it is entirely absent. Overall, cases of microtia are around 1-4 per 10,000 births with 10% having microtia in both ears. Tissue repair and replacement for the treatment of microtia can be done using two methods: autogenous torn cartilage and synthetic implants. However, this method can cause complications at the donor site and high infection rates. Based on this, a hydrogel composite made from collagen, β-TCP, and alginate is an option to be used as a scaffold in handling microtia cases. Collagen has biocompatibility and biodegradability properties which can be combined with alginate. This is because alginate can be a supporting matrix. While β-TCP is added to improve the mechanical properties. The study aimed to analyze the effect of the collagen/alginate ratio of 3:1, 2:1, and 1:1 and the addition of β-TCP of 5, 10, and 15% by weight in the hydrogel composite scaffold on morphology (SEM-EDX), properties mechanical (compression test), physical properties (swelling test), and biocompatibility (MTT assay test) for microtia reconstruction cases. The SEM results showed that there was porosity in each variation, with the largest variation in the ratio of collagen and alginate found in the 3:1 ratio with the addition of 10% β-TCP. Meanwhile, the variation in the proportion of β-TCP was found in the 15% β-TCP variation of 16.66%. In the compression test, the results of the elastic modulus values were obtained with the highest elastic modulus values in variations in the ratio of collagen and alginate 1:1 with the addition of 5% β-TCP of 1.676 ± 0.27 MPa. The maximum swelling value with variations in the addition of the proportion of β-TCP obtained the highest swelling ratio range of 249.25 ± 6.91% at the proportion of 5% β-TCP with a variation of 90 minutes. Meanwhile, the variation in the ratio of changes in collagen and alginate on 10% β-TCP resulted in a swelling ratio of 237.14 ± 10.18% at a 1:1 ratio with a variation of time of 90 minutes.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Alginate, β-TCP , Cartilage, Collagen, Microtia , Alginate, Collagen, β-TCP, Mikrotia, Tulang Rawan. |
| Subjects: | H Social Sciences > HD Industries. Land use. Labor > HD9999.C715-.C7154 Composite materials Q Science > QC Physics > QC173.4.C63 Composite materials Q Science > QP Physiology > QP34.5 Human engineering Q Science > QP Physiology > QP801.B69 Biopolymers Q Science > QP Physiology > QP801.P64 Polymers--Biodegradation T Technology > T Technology (General) > TA404 Materials--Biodegradation T Technology > TA Engineering (General). Civil engineering (General) > TA418.9 Composite materials. Laminated materials. T Technology > TP Chemical technology > TP1140 Polymers |
| Divisions: | Faculty of Industrial Technology > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Muhammad Aditya Azzamzami |
| Date Deposited: | 26 Jul 2023 01:10 |
| Last Modified: | 26 Jul 2023 01:10 |
| URI: | http://repository.its.ac.id/id/eprint/99442 |
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